![]() ![]() Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. īurnette WN (1981) “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. YALOW RS, GLICK SM, ROTH J, BERSON SA (1964) Radioimmunoassay of human plasma ACTH. Western blotting is also known as immunoblotting because it uses antibodies to detect the protein. In this method labelled antibody against particular protein is used identify the desired protein, so it is a specific test. Sarge KD, Park-Sarge O-K (2009) Detection of proteins sumoylated in vivo and in vitro. Western blotting technique is used for identification of particular protein from the mixture of protein. Biochim Biophys Acta, Mol Cell Res 1833:812–822 Voelkel T, Andresen C, Unger A et al (2013) Lysine methyltransferase Smyd2 regulates Hsp90-mediated protection of the sarcomeric titin springs and cardiac function. Pere-Brissaud A, Blanchet X, Delourme D et al (2015) Expression of SERPINA3s in cattle: focus on bovSERPINA3-7 reveals specific involvement in skeletal muscle. Proc Natl Acad Sci U S A 79:5249–5253Ĭonte A, Sigismund S (2017) Methods to investigate EGFR ubiquitination. For separation, electrophoresis in polyacrylamide gels. Wu WC, Walaas SI, Nairn AC, Greengard P (1982) Calcium/phospholipid regulates phosphorylation of a Mr “87k” substrate protein in brain synaptosomes. The numerous variations possible at each step render western blotting a versatile analytical tool. ![]()
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